Sickle Cell Disease

Comprehensive management of sickle cell disease in children

Disease Overview

Definition & Epidemiology

Sickle cell disease (SCD) is a group of inherited red blood cell disorders characterized by the presence of hemoglobin S (HbS). It affects millions of people worldwide, with the highest prevalence in sub-Saharan Africa, Mediterranean regions, and areas with historical malaria endemicity.

Global Impact

  • • ~300,000 children born with SCD annually worldwide
  • • 1 in 365 births in African Americans in the US
  • • 1 in 16,300 births in Hispanic Americans
  • • Natural selection advantage against malaria

SCD Genotypes

HbSS (Sickle Cell Anemia)

Most severe form, homozygous for HbS

HbSC Disease

Compound heterozygous, generally milder

HbS-β⁰ Thalassemia

Severe, similar to HbSS

HbS-β⁺ Thalassemia

Variable severity, some HbA present

Clinical Features

Acute Complications

  • • Vaso-occlusive crises (pain crises)
  • • Acute chest syndrome
  • • Stroke (silent or overt)
  • • Splenic sequestration
  • • Aplastic crisis
  • • Acute priapism

Chronic Complications

  • • Chronic hemolytic anemia
  • • Growth retardation & delayed puberty
  • • Chronic pain syndrome
  • • Pulmonary hypertension
  • • Chronic kidney disease
  • • Avascular necrosis
  • • Retinopathy

Functional Asplenia

  • • Increased infection risk (encapsulated bacteria)
  • • Streptococcus pneumoniae
  • • Haemophilus influenzae
  • • Neisseria meningitidis
  • • Salmonella species (osteomyelitis)

Pathophysiology

Molecular Basis

Sickle cell disease results from a single nucleotide substitution (GAG→GTG) in the β-globin gene, leading to valine replacement of glutamic acid at position 6 of the β-globin chain.

Sickling Process

1
Deoxygenation

HbS polymerizes under low oxygen conditions

2
Polymerization

Rigid polymers form, distorting RBC shape

3
Vaso-occlusion

Sickled cells block microvasculature

4
Tissue Damage

Ischemia and infarction result

Sickling Triggers

  • • Hypoxia (infection, high altitude)
  • • Dehydration
  • • Acidosis
  • • Temperature extremes
  • • Physical or emotional stress

Disease Mechanisms

Vaso-occlusion

  • • Sickled RBCs adhere to vascular endothelium
  • • Activation of coagulation cascade
  • • Inflammatory response
  • • Tissue ischemia and pain

Chronic Hemolysis

  • • Shortened RBC lifespan (10-20 days vs 120)
  • • Chronic anemia
  • • Gallstone formation
  • • Pulmonary hypertension

Organ Damage

  • • Repeated ischemia-reperfusion injury
  • • Progressive organ dysfunction
  • • Chronic pain syndromes
  • • Premature mortality

Typical Hemoglobin Levels by Genotype

Crisis Management

Vaso-occlusive Crisis

Emergency Assessment

  • • Pain location, intensity (0-10 scale)
  • • Fever, signs of infection
  • • Respiratory distress (acute chest syndrome)
  • • Neurological symptoms (stroke)
  • • Priapism in males

Pain Management Protocol

Mild Pain (1-3/10)
  • • Acetaminophen 10-15 mg/kg q4-6h
  • • Ibuprofen 5-10 mg/kg q6-8h
  • • Increased fluid intake
  • • Rest and warmth
Moderate Pain (4-6/10)
  • • Above measures PLUS
  • • Codeine 0.5-1 mg/kg q4-6h (if >12 years)
  • • Tramadol 1-2 mg/kg q6h
  • • Consider ER evaluation
Severe Pain (7-10/10)
  • Immediate ER evaluation
  • • IV morphine 0.1-0.15 mg/kg q2-4h
  • • IV fluids (1.5x maintenance)
  • • Oxygen if hypoxic
  • • Consider hospitalization

Acute Chest Syndrome

Definition

New pulmonary infiltrate on chest X-ray with ≥1 of: fever, cough, dyspnea, hypoxia, or chest pain. Medical emergency!

Clinical Presentation

  • • Fever (>38.5°C)
  • • Chest pain
  • • Cough, dyspnea
  • • Hypoxemia (O₂ sat <95%)
  • • Tachypnea, tachycardia
  • • New infiltrate on CXR

Management

Immediate Care
  • • Supplemental oxygen to maintain SpO₂ >95%
  • • IV fluids (avoid overhydration)
  • • Broad-spectrum antibiotics
  • • Incentive spirometry
Severe Cases
  • • Simple or exchange transfusion
  • • Target Hb 10-11 g/dL, HbS <30%
  • • Mechanical ventilation if needed
  • • ICU monitoring

Other Acute Complications

Splenic Sequestration

Acute spleen enlargement with ↓Hb ≥2 g/dL. Emergency transfusion needed.

Aplastic Crisis

Parvovirus B19 infection causing temporary bone marrow failure. Monitor for severe anemia.

Stroke

Emergency exchange transfusion. Consider TCD screening for prevention.

Comprehensive Management

Disease-Modifying Therapy

Hydroxyurea

First-line therapy for SCD. Increases HbF levels, reduces sickling, and decreases frequency of vaso-occlusive crises.

Indications
  • • All children with HbSS or HbS-β⁰ thalassemia ≥9 months
  • • Frequent vaso-occlusive crises
  • • History of acute chest syndrome
  • • Severe anemia
Dosing
  • • Start: 15-20 mg/kg/day
  • • Maximum: 35 mg/kg/day
  • • Monitor CBC every 2-4 weeks initially
  • • Target: HbF >20%, acceptable toxicity

Monitoring & Side Effects

  • • CBC with differential monthly
  • • Hold if ANC <2000, Hb <5.0, platelets <80,000
  • • Teratogenic - avoid pregnancy
  • • Rare: leg ulcers, nail changes

Newer Therapies

L-glutamine (Endari)

Reduces oxidative stress. For patients ≥5 years with SCD.

Crizanlizumab (Adakveo)

P-selectin inhibitor. Reduces vaso-occlusive crises frequency.

Voxelotor (Oxbryta)

Increases hemoglobin oxygen affinity, reduces sickling.

Supportive Care

Infection Prevention

Prophylactic Antibiotics
  • • Penicillin V: 2-5 years (125 mg BID), >5 years (250 mg BID)
  • • Alternative: Erythromycin or azithromycin
  • • Continue until at least age 5
Immunizations
  • • Routine childhood vaccines (no live vaccines during HU)
  • • Pneumococcal: PCV13 and PPSV23
  • • Meningococcal: all serogroups
  • • Annual influenza vaccine
  • • Hepatitis B vaccine

Chronic Management

Folic Acid Supplementation

1 mg daily for chronic hemolysis and increased folate needs.

Regular Monitoring
  • • CBC, reticulocyte count q3-6 months
  • • Comprehensive metabolic panel
  • • Liver function tests
  • • Urinalysis and microalbumin
Specialty Screening
  • • Annual TCD (2-16 years)
  • • Annual ophthalmologic exam
  • • Echocardiogram q2-3 years
  • • Pulmonary function tests

Family Education

  • • Recognize signs of complications
  • • Maintain hydration
  • • Avoid extreme temperatures
  • • Seek immediate care for fever >38.5°C
  • • Genetic counseling for family planning

Curative Therapies

Hematopoietic Stem Cell Transplant (HSCT)

Indications
  • • Recurrent vaso-occlusive crises
  • • History of stroke or acute chest syndrome
  • • Chronic organ damage
  • • Available HLA-matched sibling donor
Outcomes
  • • Overall survival: >95%
  • • Event-free survival: >90%
  • • GVHD: 15-20%
  • • Best outcomes in younger patients

Gene Therapy

Approaches
  • • Lentiviral gene addition
  • • CRISPR gene editing
  • • Autologous stem cell modification
  • • Clinical trials ongoing
Current Status
  • • Promising early results
  • • Limited long-term data
  • • High treatment costs
  • • Refer to specialized centers